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Once Bitten, Twice Shy (25 player)
Defeat Blood-Queen Lana'thel once as a vampire and again without becoming a vampire in 25-player mode.
Once Bitten, Twice Shy (25 player) requires you to defeat Blood-Queen Lana'thel twice in 25-player mode: once while carrying the Essence of the Blood Queen debuff (as a vampire), and once without ever receiving it.
Defeat Her As A Vampire
Enter the raid and let the fight begin. Blood-Queen Lana'thel applies Essence of the Blood Queen at around the 15-18 second mark — simply wait until you have that debuff, then kill her to earn credit for the vampire half of the achievement.
Defeat Her Without Vampirism
On a separate lockout, you must kill Blood-Queen Lana'thel before she can apply Essence of the Blood Queen to you, which means burning her down within roughly 15 seconds. Shamans can use their Earth Elemental to hold her attention while avoiding personal attacks that might cause her to target you for the debuff; Night Elf Druids can pull with treants and then use Shadowmeld to drop threat before the bite occurs around the 18-second mark.
Stay Alive Until Loot
You must be alive when Blood-Queen Lana'thel dies to receive achievement credit — dying even a split second after her death, such as from an enrage hit, will cause the achievement to not register.
Tips
This achievement requires two separate kills on different lockouts, so plan for at least two weekly resets to complete both halves.
See the video guide for a visual walkthrough of the solo strategy.